nucleic acid library construction protocol
Version 1

Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, starting from total RNA the mRNA is purified using polyA selection. Following chemically fragmentation the mRNA is converted into single-stranded cDNA using random hexamer priming. Next, double-stranded cDNA is generated and adapters are ligated.

SEEK ID: https://datahub-test.elixir-belgium.org/sops/80?version=1

Filename: TestProtocol_VIB.docx 

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Created: 9th Sep 2025 at 13:44

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Version 1 (earliest) Created 9th Sep 2025 at 13:44 by Flora D'Anna

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