Multi-modal plant project test
Data from Genetic properties of the MAGIC maize population: a new platform for high definition QTL mapping in Zea mays https://doi.org/10.1186/s13059-015-0716-z
Web page: Not specified
Funding details: Related items
Projects: ISA ENA export, testing template created by user in SP, Second project, Genetic properties of the MAGIC maize population, CODEX on TMA from metastatic uveal melanoma biopsies, Test project seek-1.15, WP2 - Bioprospecting and functional expression of CYPs, WP1 - Learning techniques fit for industrial biotechnology, deCYPher Composable Elements, deCYPher experiments templates
Institutions: Default Institution, Vlaams Instituut voor Biotechnologie, uppsala University, ILVO
Test for MARS
Programme: Multi-modal plant project test
Public web page: Not specified
WGS, RNAsequencing and genotyping of founders lines
Genome-seq_RNAseq_variants of founders lines
Submitter: Flora D'Anna
Investigation: Characterisation of founders lines
Assays: Genome Assembly, Genome Variants, Genome sequencing, Genomic, Genomic variants, Genomic variants, Library preparation, Library preparation, RNA extraction, RNA sequencing, Transcriptomics
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Genomic variants
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Genome assembly protocol
Creator: Flora D'Anna
Submitter: Flora D'Anna
Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, starting from total RNA the mRNA is purified using polyA selection. Following chemically fragmentation the mRNA is converted into single-stranded cDNA using random hexamer priming. Next, double-stranded cDNA is generated and adapters are ligated.
Creator: Flora D'Anna
Submitter: Flora D'Anna
Total RNA was extracted using TriZol.
Creator: Flora D'Anna
Submitter: Flora D'Anna
The basal 5 millimeter of the fourth leaf was sampled for RNA extraction harvested two days after its appearance from the surrounding older leaves.
Creator: Flora D'Anna
Submitter: Flora D'Anna
Plants were grown in the growth chamber under controlled growth conditions (24oC, 55% relative humidity, 170 mmol m-2 s-1 photosynthetically active radiation at plant level in a 16-h/8-h day/night cycle).
Creator: Flora D'Anna
Submitter: Flora D'Anna
