SEEK ID: https://datahub-test.elixir-belgium.org/people/3
Locations:
United Kingdom
,
Belgium
,
Sweden
ORCID: Not specified
Joined: 18th Jul 2022
Expertise: Not specified
Tools: Not specified
Roles
Admin
Project administrator
- ISA ENA export
- testing template created by user in SP
- Second project
- Genetic properties of the MAGIC maize population
- WP1 - Learning techniques fit for industrial biotechnology
- WP2 - Bioprospecting and functional expression of CYPs
- deCYPher Composable Elements
- deCYPher experiments templates
Programme administrator
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Related items
- Programmes (6)
- Projects (11)
- Institutions (5)
- Investigations (1+2)
- Studies (1+1)
- Assays (11+5)
- SOPs (6+2)
- Publications (1)
- Sample types (10+17)
- Samples (34+12)
- Experiment Sample Templates (3+4)
Projects: Templates - EGA Data Access Committee @ VIB
Web page: Not specified
Data from Genetic properties of the MAGIC maize population: a new platform for high definition QTL mapping in Zea mays https://doi.org/10.1186/s13059-015-0716-z
Projects: Genetic properties of the MAGIC maize population
Web page: Not specified
Test
Projects: WP2 - Bioprospecting and functional expression of CYPs, deCYPher Composable Elements, deCYPher experiments templates, WP1 - Learning techniques fit for industrial biotechnology, WP4 - Sustainable bio-based production of flavonoids and terpenoids, WP3 - MCFs for flavonoids and terpenoids
Web page: https://www.decypher.bio/
Projects: Kevin's Test project, Project Test Permissions, Test program seek-1.14, Test Project 07-11-2023, Test project seek-1.15, ENA Upload tool
Web page: Not specified
Projects: testing template created by user in SP, Second project, Test project Kevin
Web page: Not specified
Programme: EGA Data Access Committee @ VIB
Public web page: Not specified
Organisms: Not specified
Test for MARS
Programme: Multi-modal plant project test
Public web page: Not specified
Organisms: Not specified
Templates to be used by wet-lab when performing an experiment for a WP or Task to describe experimental conditions in Study and Assays.
Programme: deCYPher
Public web page: Not specified
Organisms: Not specified
Tables as defined by the data model and the codebook
Programme: deCYPher
Public web page: Not specified
Organisms: Not specified
Objectives: WP 2 main’s objective is to overcome the challenge to efficiently identify, select and functionally express CYPs in microbial hosts by: (i) Better understanding of the CYP structure/product specificity relationship (SO1.2/SO3.2) (ii) Correct localisation & functional expression of CYPs in different microbial hosts (SO1.3) (iii) Resolving stress relief caused by CYP expression in microbes (SO2.1).
Programme: deCYPher
Public web page: Not specified
Organisms: Not specified
ROR ID: Not specified
Department: Not specified
Country:
United Kingdom
City: Not specified
Web page: Not specified
ROR ID: Not specified
Department: Not specified
Country:
Belgium
City: Not specified
Web page: Not specified
ROR ID: Not specified
Department: Not specified
Country:
Sweden
City: Stockholm
Web page: Not specified
ROR ID: https://ror.org/03xrhmk39
Department: Not specified
Country:
Belgium
City: Ghent
Web page: https://vib.be/
WGS, RNAsequencing and genotyping of founders lines
Submitter: Flora D'Anna
Studies: Genome-seq_RNAseq_variants
Assays: Genome Assembly, Genome Variants, Genome sequencing, Genomic, Genomic variants, Genomic variants, Library preparation, Library preparation, RNA extraction, RNA sequencing, Transcriptomics
Snapshots: No snapshots
Genome-seq_RNAseq_variants of founders lines
Submitter: Flora D'Anna
Investigation: Characterisation of founders lines
Assays: Genome Assembly, Genome Variants, Genome sequencing, Genomic, Genomic variants, Genomic variants, Library preparation, Library preparation, RNA extraction, RNA sequencing, Transcriptomics
Snapshots: No snapshots
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Organisms: No organisms
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Organisms: No organisms
SOPs: nucleic acid extraction protocol
Data files: No Data files
Snapshots: No snapshots
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Organisms: No organisms
SOPs: No SOPs
Data files: No Data files
Snapshots: No snapshots
Genomic variants
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Organisms: No organisms
SOPs: nucleic acid library construction protocol
Data files: No Data files
Snapshots: No snapshots
Genome assembly protocol
Creator: Flora D'Anna
Submitter: Flora D'Anna
Investigations: Characterisation of founders lines
Studies: Genome-seq_RNAseq_variants
Assays: Genomic variants
Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, starting from total RNA the mRNA is purified using polyA selection. Following chemically fragmentation the mRNA is converted into single-stranded cDNA using random hexamer priming. Next, double-stranded cDNA is generated and adapters are ligated.
Creator: Flora D'Anna
Submitter: Flora D'Anna
Investigations: Characterisation of founders lines
Studies: Genome-seq_RNAseq_variants
Assays: Library preparation
Total RNA was extracted using TriZol.
Creator: Flora D'Anna
Submitter: Flora D'Anna
Investigations: Characterisation of founders lines
Studies: Genome-seq_RNAseq_variants
Assays: RNA extraction
The basal 5 millimeter of the fourth leaf was sampled for RNA extraction harvested two days after its appearance from the surrounding older leaves.
Creator: Flora D'Anna
Submitter: Flora D'Anna
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Plants were grown in the growth chamber under controlled growth conditions (24oC, 55% relative humidity, 170 mmol m-2 s-1 photosynthetically active radiation at plant level in a 16-h/8-h day/night cycle).
Creator: Flora D'Anna
Submitter: Flora D'Anna
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Abstract (Expand)
Authors: M. Dell'Acqua, D. M. Gatti, G. Pea, F. Cattonaro, F. Coppens, G. Magris, A. L. Hlaing, H. H. Aung, H. Nelissen, J. Baute, E. Frascaroli, G. A. Churchill, D. Inze, M. Morgante, M. E. Pe
Date Published: 11th Sep 2015
Publication Type: Journal Article
PubMed ID: 26357913
Citation: Genome Biol. 2015 Sep 11;16(1):167. doi: 10.1186/s13059-015-0716-z.
Test for MARS
Input (Sample Name) (Registered Sample List) *, Analysis Title (String) *, Analysis Alias (String) *, Description (String) *, Experiment Type (Controlled Vocabulary - EVA Experiment Type CV) *, Reference (String) *, Raw Data File (String) *, File path (String) , Checksum type (String) , Checksum (String)
Not specified
Test for MARS
Input (FASTA) (Registered Sample List) *, Analysis Title (String) *, Analysis Alias (String) *, Description (String) *, Experiment Type (Controlled Vocabulary - EVA Experiment Type CV) *, Reference (String) *, Data file Name (String) *
Not specified
Genome assembly
Input (Raw Data File) (Registered Sample List) *, sequence assembly protocol (String) *, FASTA (String) *, FASTA URL (String) , ASSEMBLYNAME (String) *, ASSEMBLY_TYPE (Controlled Vocabulary - ENA ASSEMBLY_TYPE) *, COVERAGE (String) *, PROGRAM (String) *, PLATFORM (String) *, MOLECULETYPE (String)
Not specified
Input (library name) (Registered Sample List) *, nucleic acid sequencing (String) *, instrument_model (Controlled Vocabulary - sequencing instrument (free text allowed)) *, file type (Controlled Vocabulary - file type (free text allowed)) *, Raw Data File (String) *, MD5 (String)
Not specified
Genomic library preparation
Input (Sample Name) (Registered Sample List) *, library source (Controlled Vocabulary - library source (free text allowed)) *, library strategy (Controlled Vocabulary - library strategy (free text allowed)) *, library selection (Controlled Vocabulary - library selection (free text allowed)) *, library layout (Controlled Vocabulary - library layout (free text allowed)) *, library name (String) *, library construction (String) *
Not specified
Genome assembly
Input (Registered Sample List) *, sequence assembly protocol (String) *, FASTA (String) *, FASTA URL (String) , ASSEMBLYNAME (String) *, ASSEMBLY_TYPE (Controlled Vocabulary - ENA ASSEMBLY_TYPE) *, COVERAGE (String) *, PROGRAM (String) *, PLATFORM (String) *, MOLECULETYPE (String)
Test for MARS
Input (Registered Sample List) *, Analysis Title (String) *, Analysis Alias (String) *, Description (String) *, Experiment Type (Controlled Vocabulary - EVA Experiment Type CV) *, Reference (String) *, Data file Name (String) *
Input (Registered Sample List) *, library construction (String) *, library_construction_protocol (String) , title (String) *, design_description (String) , library_name (String) *, library_source (Controlled Vocabulary - library_source) *, library_strategy (Controlled Vocabulary - library_strategy) *, library_selection (Controlled Vocabulary - library_selection) *, library_layout (Controlled Vocabulary - library_layout) *, insert_size (String) , platform (Controlled Vocabulary - platform) *, instrument_model (Controlled Vocabulary - instrument_model) *, accession (String) , submission_date (String) , status (Controlled Vocabulary - status)