Characterisation of founders lines
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Order Studies WGS, RNAsequencing and genotyping of founders lines
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Creators and SubmitterViews: 141
Created: 5th Sep 2025 at 16:50
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Institutions: Default Institution, Vlaams Instituut voor Biotechnologie, uppsala University, ILVO
Data from Genetic properties of the MAGIC maize population: a new platform for high definition QTL mapping in Zea mays https://doi.org/10.1186/s13059-015-0716-z
Projects: Genetic properties of the MAGIC maize population
Web page: Not specified
Test for MARS
Programme: Multi-modal plant project test
Public web page: Not specified
Genome-seq_RNAseq_variants of founders lines
Submitter: Flora D'Anna
Investigation: Characterisation of founders lines
Assays: Genome Assembly, Genome Variants, Genome sequencing, Genomic, Genomic variants, Genomic variants, Library preparation, Library preparation, RNA extraction, RNA sequencing, Transcriptomics
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Genomic variants
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Submitter: Flora D'Anna
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Genome assembly protocol
Creator: Flora D'Anna
Submitter: Flora D'Anna
Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, starting from total RNA the mRNA is purified using polyA selection. Following chemically fragmentation the mRNA is converted into single-stranded cDNA using random hexamer priming. Next, double-stranded cDNA is generated and adapters are ligated.
Creator: Flora D'Anna
Submitter: Flora D'Anna
Total RNA was extracted using TriZol.
Creator: Flora D'Anna
Submitter: Flora D'Anna
The basal 5 millimeter of the fourth leaf was sampled for RNA extraction harvested two days after its appearance from the surrounding older leaves.
Creator: Flora D'Anna
Submitter: Flora D'Anna
