RNA sequencing
Experiment ViewSEEK ID: https://datahub-test.elixir-belgium.org/assays/219
Experimental assay
Projects: Genetic properties of the MAGIC maize population
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Assay position: 2
Organisms: No organisms
Is ISA-JSON compliant: true
- nucleic acid sequencing 1.2.0 (id: 10)
Assay Stream: Transcriptomics
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Creators and SubmitterViews: 171
Created: 9th Sep 2025 at 15:11
Last updated: 9th Sep 2025 at 15:31
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Institutions: Default Institution, Vlaams Instituut voor Biotechnologie, uppsala University, ILVO
Data from Genetic properties of the MAGIC maize population: a new platform for high definition QTL mapping in Zea mays https://doi.org/10.1186/s13059-015-0716-z
Projects: Genetic properties of the MAGIC maize population
Web page: Not specified
Test for MARS
Programme: Multi-modal plant project test
Public web page: Not specified
WGS, RNAsequencing and genotyping of founders lines
Genome-seq_RNAseq_variants of founders lines
Submitter: Flora D'Anna
Investigation: Characterisation of founders lines
Assays: Genome Assembly, Genome Variants, Genome sequencing, Genomic, Genomic variants, Genomic variants, Library preparation, Library preparation, RNA extraction, RNA sequencing, Transcriptomics
Input (library name) (Registered Sample List) *, sequencing instrument (Controlled Vocabulary - sequencing instrument (free text allowed)) *, Assay Name (String) *, Technology type (Controlled Vocabulary - Technology type (free text allowed)) *, FASTQ_URI (String) *, nucleic acid sequencing (String) *, Performer (String) , Scan name (String) , Submitted File Name (String) , MD5 (String) , SPOT_LENGTH (String) , READ_INDEX_1_BASE_COORD (String)
Not specified
nucleic acid sequencing protocol Version 1
Clusters are generated through amplification using the TruSeq PE Cluster Kit v3-cBot-HS kit (Illumina) followed by sequencing on a Illumina HiSeq2000 using the TruSeq SBS Kit v3-HS (Illumina). Sequencing was performed in Paired-End mode with a read length of 100 b.clusters are generated through amplification using the TruSeq PE Cluster Kit v3-cBot-HS kit (Illumina) followed by sequencing on a Illumina HiSeq2000 the TruSeq SBS Kit v3-HS (Illumina). Sequencing was performed in Paired-End mode with a read length of 100 nt.
SEEK ID: https://datahub-test.elixir-belgium.org/sops/81
Link: /assays/219
Filename: TestProtocol_VIB.docx
Format: Word document
Size: 13.2 KB
