Library preparation
Experiment ViewSEEK ID: https://datahub-test.elixir-belgium.org/assays/218
Experimental assay
Projects: Genetic properties of the MAGIC maize population
Investigation: Characterisation of founders lines
Study: Genome-seq_RNAseq_variants
Assay position: 1
Organisms: No organisms
Is ISA-JSON compliant: true
- library construction 1.2.0 (id: 8)
Assay Stream: Transcriptomics
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Creators and SubmitterViews: 262
Created: 9th Sep 2025 at 13:37
Last updated: 9th Sep 2025 at 13:50
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Projects: ISA ENA export, testing template created by user in SP, Second project, Genetic properties of the MAGIC maize population, CODEX on TMA from metastatic uveal melanoma biopsies, Test project seek-1.15, WP2 - Bioprospecting and functional expression of CYPs, WP1 - Learning techniques fit for industrial biotechnology, deCYPher Composable Elements, deCYPher experiments templates, Templates - EGA Data Access Committee @ VIB, Example of HoloFood experiments template, Croockshanks' PhD, EGA submissions - H. Granger Lab, EGA submissions - H. Potter Lab, HoloFood in DataHub
Institutions: Default Institution, Vlaams Instituut voor Biotechnologie, uppsala University, ILVO, VIB, Data Core, Vlaams Instituut voor Biotechnologie
Data from Genetic properties of the MAGIC maize population: a new platform for high definition QTL mapping in Zea mays https://doi.org/10.1186/s13059-015-0716-z
Projects: Genetic properties of the MAGIC maize population
Web page: Not specified
Test for MARS
Programme: Multi-modal plant project test
Public web page: Not specified
WGS, RNAsequencing and genotyping of founders lines
Genome-seq_RNAseq_variants of founders lines
Submitter: Flora D'Anna
Investigation: Characterisation of founders lines
Assays: Genome Assembly, Genome Variants, Genome sequencing, Genomic, Genomic variants, Genomic variants, Library preparation, Library preparation, RNA extraction, RNA sequencing, Transcriptomics
Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, starting from total RNA the mRNA is purified using polyA selection. Following chemically fragmentation the mRNA is converted into single-stranded cDNA using random hexamer priming. Next, double-stranded cDNA is generated and adapters are ligated.
Creator: Flora D'Anna
Submitter: Flora D'Anna
Input (Extract Name) (Registered Sample List) *, library source (Controlled Vocabulary - library source (free text allowed)) *, library strategy (Controlled Vocabulary - library strategy (free text allowed)) *, library selection (Controlled Vocabulary - library selection (free text allowed)) *, library layout (Controlled Vocabulary - library layout (free text allowed)) *, library strand (Controlled Vocabulary - library strand (free text allowed)) , orientation (Controlled Vocabulary - orientation (free text allowed)) , library name (String) *, library construction (String) *, Nominal Length (Integer) , Nominal SDev (Real number)
Not specified
nucleic acid library construction protocol Version 1
Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, starting from total RNA the mRNA is purified using polyA selection. Following chemically fragmentation the mRNA is converted into single-stranded cDNA using random hexamer priming. Next, double-stranded cDNA is generated and adapters are ligated.
SEEK ID: https://datahub-test.elixir-belgium.org/sops/80
Link: /assays/218
Filename: TestProtocol_VIB.docx
Format: Word document
Size: 13.2 KB
